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RNAscope®Multiplex Fluorescent Assay v2(PN 323110)
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  • 产品编号:
  • 产品名称:RNAscope®Multiplex Fluorescent Assay v2(PN 323110)
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Introducing this new TSA-based assay that enables single-molecule detection of up to four RNA targets simultaneously. The significant signal boost provided by simultaneous background noise suppression from ACD’s patented signal amplification technology combined with Perkin Elmer’s TSA® technology allows expanded applications such as dual ISH-IHC and support of FFPE samples.


Technology Overview:

RNAscope® Multiplex Fluorescent Assay v2 allows simultaneous detection of up to four RNA targets within a single sample using up to four target probes and the nuclei can be counter stained and visualized using DAPI fluorescent dye. The four target probes, each designed a different probe channel—C1, C2, C3, or C4 contain several components. The lower part of the double Z probe is designed to hybridize to a specific RNA target. The upper part of the double Z probe, along with the preamplifiers, amplifiers and dyes are assigned to a specific probe channel. For 4-plex assay we recommend using the Opal 520 (FITC range), Opal 570 (Cy 3 Range), Opal 620 (Texas Red Range) and Opal 690 (Cy 5 Range) dyes. The sequential assay workflow provides flexibility to visualize any probe in any of the TSA linked fluorophore channels.




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